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The biological effects of mineral-forming matrix vesicles (MVs) from different stages of maturation of rat kidney were evaluated by single and mixed protein gel electrophoresis. A calibration curve has been obtained from the protein profile of MVs, which enables the use of this technique for the determination of MVs concentration. The protein/DNA ratio, as well as the protein composition (insulin-like growth factor-binding protein-2, bone morphogenetic protein-2, alkaline phosphatase) remained constant during renal maturation. The results showed that the mechanisms of renal inorganic phosphate (Pi) transport are formed at the time of initiation of excretory functions and are not altered at later stages of nephrogenesis. The concentrations of total MVs isolated from different stages of development exhibit a marked increase, from 8.2+/-0.5 x 10(9) MVs/g wet tissue at 10 days of age to 9.5+/-0.6 x 10(9) MVs/g wet tissue at the end of the second week of life (PQ:
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